ELISA Electrophoresis System (Transfer)-Gel to Blot Transer System (for Western Blot)
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Product introduction
This product can be used with SVE-2 vertical electrophoresis. The electric field is used to transfer the proteins, nucleic acids, etc. in the gel after electrophoresis to the transfer membrane, and then the charged particles are further analyzed and studied by means of biochemical analysis.
Product Features
a. Double plate structure, can be used with SVE-2 electrophoresis tank.
b. The tank of the instrument is integrated injection molding with high transparency polycarbonate, it's corrosion resistance, high transparency, consistent performance, etc..
c. The positive and negative electrodes of the transfer clip are distinct, with a handle function, which is easy to operate.
d. High transfer efficiency, transfer time is about 20-40min.
e. Strong structure can be synchronized with the ice bath cooling to avoid interference caused by overheating, so as to ensure reliable experimental resµLts.
Product Specifications
SVE-2
SVT-2
Dimensions (L*W*H) mm
185x108x125
185x108x125
Gel Quantity/transfer Quantity (mm)
Gel Quantity 83x73
Transfer Quantity 70x90
Maximum volume of buffer (mL)
500
500
Operation process
4.1 Preparation
Prepare 8 pieces of 7x9cm thin transfer membrane filter paper or 2 pieces of thick filter paper of the same specification in advance, and 1 piece of 7x9cm PVDF membrane (currently, the membrane with a pore Quantity of 0.45μm is used for proteins greater than 20kda, and the membrane with a pore Quantity of 0.2μm is used for proteins less than 20kda).
4.2 Activation
The PVDF membrane needs to be activated with methanol for 2 min before use.
4.3 Installation
According to the order of ‖ positive electrode (+) > sponge > filter paper > transfer film > gel > filter paper > sponge > negative electrode (-) ‖, place the transfer material in the transfer clip and remove the air bubbles. The red side is the positive direction, and the black side is the negative side. Place the transfer clip into the transfer core in the direction of red to red and black to black. The transfer core can hold two transfer clips at the same time.
4.4 Power-on transfer
Connect the electrophoresis power supply, set the transfer conditions, and start transferring the membrane.
4. Product configuration list
Serial number
Name
Quantity
1
transfer clip
2 sets
2
transfer core
1 set
3
electrophoresis box
1 set
4
Transfer sponge
1 box
5
Transfer filter paper
1 box
Precautions
1. The main components of this series of products are fragile products. During packaging, transportation and use, be carefµL not to bump or drop the product, which may cause damage to the product and cause the product to fail to work normally.
2. Metal parts such as platinum wire and electrode column in this product shoµLd be paid attention to whether there are oxidation, breakage, loosening and other problems during use, cleaning and maintenance. Please replace and maintain them in time. The platinum wire is easily broken, so be carefµL when handling it.
3. After daily use, please wash it with deionized water in time, and place it in a dust-free place to dry for use.
Return to the list
Product recommendation
Cat.No.
Product Name
Spec.
Operation
G6001-16
Transfer Membrane Filter Paper (thick)
16 pcs(7*9 cm)
G6001-200
Transfer Membrane Filter Paper (Thick)
200pcs(7*9 cm)
G6002
Transfer Sponge
2 pcs/box
G6002-15
Transfer Sponge
15pcs(7*9 cm)
G6007-50
Transfer Membrane Filter Paper (thin)
50 pcs/bag
G6007-500
Transfer Membrane Filter Paper (thin)
500 pcs/box
G6014-15*15CM
NC Membrane (NitrocellµLose Membrane)
15*15cm
G6015-0.45
PVDF Membrane (0.45 μm)
0.45 μm 13.25*15cm
G6015-1-0.22
PVDF Membrane (0.2μm)
0.2 μm 13.25*15cm
G6018-15*15CM
Nylon Membrane
15 cm*15 cm
G6019-9
Antibody Incubation Box (9 Grids)
9 grids
G6020-9
Antibody Incubation Box (9 Grids Light-Proof)
9 Grids Light-Proof
G6025-2
Antibody Incubation Box (2 Grids)
2 grids
G6026-4
Antibody Incubation Box (4 Grids)
4 grids
G6044-0.45
PVDF Membrane(0.45μm)
0.45 μm 7*9cm 10 pcs/bag
G6045-0.22
PVDF Membrane (0.22μm)
0.2μm 7*9 cm 10 pcs/bag
G6046-0.2
PVDF Transfer Membranes(0.2μm, 5.5×8.5cm)
0.2 μm 5.5*8.5 cm 10 sheets per pack.
G6047-0.45
PVDF Transfer Membranes(0.45μm, 5.5×8.5cm)
0.45 μm 5.5*8.5 cm 10 sheets per pack.
G6048-2
WB Transfer Sponge (Filter Paper-Free)
2pcs(7*9 cm)
G6072-2
Antibody Incubation Box (2 Grids Light-Proof)
2 Grids Light-Proof
G6073-4
Antibody Incubation Box (4 Grids Light-Proof)
4 Grids Light-Proof
G6075-4
Antibody Incubation Box (4-grid, Light-tight)
4-grid, Light-tight
G9055-1
Antibody Incubation Box (1 Grid FµLl Membrane)
1 Grid FµLl Membrane
G9055-4
Antibody Incubation Box (4 Grids FµLl Membrane)
4 Grids FµLl Membrane
SPW-6S
Electrophoresis Power Supply (Programmable)
Set
SVT-2
Electrophoresis System (Transfer)
WGP-66485
NC Membrane (NitrocellµLose Membrane)
30 cm*3 m
WGPVDF22
PVDF Membrane (0.2μm)
0.2 μm 26.5 cm*3.75 m
WGPVDF45
PVDF Membrane(0.45μm)
0.45 μm 26.5 cm*3.75 m
Internal Reference:
SVT-2
Website URL:
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