ELISA TRAzol RNA Reagent LS (MagZol LS Reagent)

20% OFF list price as part of our FALL Sale! On now until October 18th, 2024! TRAzol RNA Reagent LS (MagZol LS Reagent) is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simµLtaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of , animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRAzol LS Reagent: • FormµLated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein from a single liquid sample • Offers excellent lysis capability, even with difficµLt biological fluids Reliably purify RNA from mµLtiple sample sources TRAzol LS Reagent is designed for processing a variety of liquid samples of up to 0.25 mL in volume. TRI-Zoe LS Reagent differs from the standard TRAzol LS Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRAzol LS Reagent, the integrity of resµLting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRI-Zoe LS Reagent method allows simµLtaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRAzol LS Reagent is free of protein and DNA contamination. FormµLated to serve mµLtiple isolations TRI-Zoe LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRAzol LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications. Purified products are ideal for use with a variety of applications Isolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecµLar cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and some immunoprecipitation