ELISA HiPure Plant RNA Mini

20% OFF list price for our Summer Sale! On now until JµLy 31, 2024! Introduction The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient µLtracentrifµgation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation. Details Specifications Features Specifications Main Functions Isolation total RNA from 150 mg plant using two columns (gDNA removed column) Applications RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation Purification method Mini spin column Purification technology Silica technology, DNA filtration technology Process method Manual (centrifµgation or vacuum) Sample type Economic crops Sample amount ≤150 mg Elution volume ≥30μl Time per run ≤25 minutes Liquid carrying volume per column 800µl Binding yield of column 100µg Principle The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed throµgh a DNA Mini column, ethanolis added to the flow-throµgh, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. Advantages Efficient removal of DNA - unique genomic DNA removal column without DNase treatment High quality - high purity total RNA can be directly used in various sensitive downstream applications Fast - several samples can be extracted in 25 minutes by column method Safe - no phenol chloroform extraction required Sensitive - RNA can be recovered at the level of PG Broad spectrum - various types of plant samples can be processed by diversity of operating procedures Kit Contents Contents R415102 D415103 Purification Times 50 Preps 250 Preps HiPure DNA Mini Column II 50 250 HiPure RNA Mini Columns 50 250 2mL Collection Tubes 100 2 x 250 Buffer RLC 50 mL 200 mL Buffer PRC1 50 mL 200 mL Buffer RW1 50 mL 200 mL Buffer RW2* 20 mL 2 x 50 mL RNase Free Water 10 mL 30 mL Storage and Stability The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.