ELISA Pfu DNA Polymerase 2x Master Mix

Pfu DNA polymerase 2x master mix, the same high performance enzyme with the convenience of master mixes. Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions resµLts in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis. Components: 2x Pfu DNA polymerase master mix Available in two Quantitys, 1 mL and 5 mL. Features 3'-5' exonuclease activity provides a low error rate One of the most thermostable DNA polymerases known Lack of extendase activity means no unwanted 3’ overhangs Optimal for blunt-ended PCR cloning Optimum temperature near 75°C 95% active after 1hour incubation at 98°C Applications High-fidelity PCR and primer-extension reactions PCR cloning and blunt-ended amplification product generation