ELISA VAHTS Universal V8 RNA-seq Library Prep for Illumina

20% OFF list price for the 24 reaction variant as part of our FALL Sale! On now until October 18th, 2024! The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-Seq transcriptome analysis. This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific Quantitys, which can be customized, can be obtained after Quantity selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability. VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina is suitable for RNA library construction of RNA that have been enriched by Poly(A) (for RNA with good integrity from eukaryotes such as animals, plants and fungi) or rRNA depletion. As the content of mRNA in total RNA of different samples varies greatly, enoµgh total RNA need to be inputted to make sure the sufficient mRNA for library construction. The amount of input-RNA is related to the mRNA enrichment modµLe: VAHTS mRNA Capture Beads (Vazyme #N401): 0.01 - 4 μg; Ribo-off rRNA Depletion Kit (H/R/M) (Vazyme #N406): 0.01 - 1 μg; Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407): 0.01 - 5 μg; Ribo-off Globin & rRNA Depletion Kit (H/M/R) (Vazyme #N408): 0.01 - 1μg; Ribo-off rRNA Depletion Kit (Plant) (Vazyme #N409): 1 - 5 μg; Purified mRNA or Ribosomal-depleted RNA: 0.5 - 100 ng; It is recommended to use Agilent 2100 Bioanalyzer to analyze the integrity of total RNA. mRNA enriched with VAHTS mRNA Capture Beads (Vazyme #N401) must be high quality RNA (RIN ≥ 7). Degraded total RNA used for library construction will lead to 3’ bias in RNA-seq. For RNA samples with RIN value < 7, rRNA removal can be performed using the Ribo-off method (Vazyme #N406/407/408/409). Main fields of RNA-related analysis: ◇ gene expression analysis ◇ single nucleotide variation calling