ELISA VAHTS Stranded mRNA-seq Library Prep for Illumina

Kit composition • Precise measurement of strand Orientation • Ideal Kit for complex plant template • Identical protocol with Illumina TruSeq Stranded mRNA Sample Prep Kit • Optimization for large fragments • High consistency with Illumina reagent. VAHTS Stranded mRNA-seq Library Prep Kitfor Illumina® is designed for strand specific transcriptome library preparation, ideal for high throµghput sequencing. The stranded kit is different from other library construction methods because dUTP is already included in the 2 nd strand cDNA synthesis and is digested by uracil-DNA glycosylase(UDG) prior to PCR amplification. As a resµLt, only information from the 1 st strand cDNA is preserved. Data analysis of highthroµghput sequencing will provide strand specific (i.e. from sense or anti-sense DNA) information in addition to standard transcriptome information.