ELISA VAHTS® RNA Multi-PCR Library Prep with SARS-CoV-2 Panel

Two rounds of PCR amplification are adopted to complete target amplification and enrichment. It only takes 5 h from viral genomic sample acquisition to obtaining complete sequencing library, and the manual operation time is less than 1.5 hours. This product is compatible with the starting template range of 16 Ct to 38 Ct, and the constructed library is suitable for sequencing analysis on the Illumina platform. The customized panel in this kit has a coverage of 99.7% of the whole genome. With mµLti-PCR amplification reagents, the uniformity can reach 90%, which can effectively detect the mutation and deletion sites. The optimized combination of the reagents enables easy, stable and reliable RNA library construction. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the optimal stability and repeatability of library construction. Components Components NA211-01 NA211-02 (24 rxns) (96 rxns)  10× RT Mix 48μl 192 μl  HiScriptIII Enzyme Mix 48μl 192 μl  RandomHexamers 24μl 96μl  RNase-freeddH2O 1 mL 1 mL  4 × VAHTSMµLti-PCR Mix 120μl 480 μl  Primer Pool1 24μl 48μl  Primer Pool2 24μl 48μl  VAHTSHiFiAmplificationMix 960μl 4 × 960 μl  TE 1 mL 4 × 1 mL ▲Refer to Appendix for the second round PCR primers. Panel (V3.0) information: Number of pairs of primers: 247 Pool 1 primer pairs: 125 Pool 2 primer pairs: 122 Net product Quantity range: 54-232 bp Product Quantity range (including library sequence): 191-369 bp Cross-compatible with known variants of concerns Genomic coverage: >99.7%