ELISA T7 Endonuclease I

Product Details T7 Endonuclease I is a junction-resolving enzyme of 149 amino acid residue, encoded by gene 3 of bacteriophage T7, existing as a stable dimer. Not only does it selectively bind and cleave four-way DNA (Holliday) junctions with high specificity for branched structures in double-stranded DNA, such as cruciform DNA, but also it has a strong preference for cutting single-stranded DNA. It requires metal ions such as magnesium for activity. This product is purified from E. coli expressing the recombinant T7 Endonuclease I (T7EI) gene. Definition of Activity Unit One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C. Applications • Gene mutation and SNP detection for resµLt from TALEN and CRISPR/CAS9 • Recognition and cleavage for non-perfectly matched DNA and Holliday junctions • RandomLy cleave single-stranded DNA Storage at -20°C for one year Shipping Dry ice (-70 ℃) Product Contents Component LE101-01 LE101-02 T7 Endonuclease I 250 units 5×250 units 10×T7 Endonuclease I Buffer 200 μl 1 mL T7 Endonuclease I Control Template (40 ng/μl) 20 μl 20 μl 10×DNA Loading Buffer 1 mL 1 mL