ELISA TransNGSTM rRNA Depletion (-Mouse-Rat)

Description TransNGS™ rRNA Depletion Kit (/Mouse/Rat) depletes ribosomal RNA from /mouse/rat total RNA by RNase H digestion, while retains messenger RNA and non-coding RNA. The depleted ribosomal RNA contains both cytoplasmic ribosomal RNA (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA). The resµLting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis. Features: • Remove up to 99% ribosomal RNA from /mouse/rat total RNA. • Control qPCR Primer Sets are provided to monitor the depletion efficiency of ribosomal RNA and the retention rate of non-ribosomal RNA. Storage at-20°C for one year Kit Content Component KD101-01 (6 rxns) KD101-02 (24 rxns) KD101-03 (96 rxns) rRNA Probe (H/M/R) 12 μl 48 μl 192 μl E.coli RNase H 6 μl 24 μl 96 μl DNase I 30 μl 120 μl 480 μl 5×Hybridization Buffer 18 μl 72 μl 288 μl 10×RNase H Reaction Buffer 12 μl 48 μl 192 μl 10×DNase I Reaction Buffer 30 μl 120 μl 480 μl RNase-free Water 500 μl 2×1 mL 8×1 mL Control qPCR Ribosomal RNA Primer Mix 12 μl 48 μl 192 μl Non-ribosomal RNA Primer Mix 12 μl 48 μl 192 μl