ELISA T7 High Efficiency Transcription

Description T7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. Up to 150 μg of RNA can be produced from a 20 μl reaction. SyntheQuantityd RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays. Storage at -20°C for one year Kit Content Component JT101-01 (25 rxns) T7 Transcription Enzyme Mix 50 µl 5×T7 Transcription Reaction Buffer 100 µl 10 mM NTP Mix 200 µl DNase I (1 unit/µl) 25 µl 500 mM EDTA (pH 8.0) 25 µl RNase-free Water 500 µl Control Transcription Template (0.5 µg/µl) 10 µl