ELISA FlyCut® SacII

Product Details FlyCut® SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molecµLar weight is 32.1 kDa, with the recognition site at CCGC^GG The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation. Recognition Site 5’...CCGC↓GG...3’ 3’...GG↑CGCC...5’ Applications Genomic DNA, plasmid DNA, PCR product Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component JS201-01 JS201-02 FlyCut® SacII 1,000 units 2×1,000 units 10×FlyCut® Buffer 250 μl 500 μl 10×DNA Loading Buffer 1 mL 1 mL