ELISA FlyCut® EagI

Product Details FlyCut® EagI is expressed and purified from E.coli that carries the recombinant EagI gene. The molecµLar weight is 35.1 kDa, with the recognition site at C^GGCCG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation. Recognition Site 5’...C↓GGCCG...3’ 3’...GCCGG↑C...5’ Applications Genomic DNA, plasmid DNA, PCR product Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component JE101-01 JE101-02 FlyCut® EagI 250 units 2×250 units 10×FlyCut® Buffer 250 μl 250 μl 10×DNA Loading Buffer 1 mL 1 mL