Vaccinia Capping Enzyme, GMP Grade - 500 KU

The vaccinia capping enzyme together with substrates, is capable to add the 7-methylguanosine capping structure (m7Gppp, Cap0) to the 5´ end of the RNA. In eukaryotes, this structure is closely related to the stability, transport, and translation of mRNA. Capping RNA with an enzymatic reaction is a simple and effective method that can significantly improve the stability and translation capabilities of RNA used for in vitro transcription, transfection, and micro-injection. The vaccinia virus capping enzyme consists of two subunits (D1 and D12) (Figure 1a), and possesses RNA triphosphatase, guanosine transferase, and guanine methyltransferase activities, and they are all necessary for adding a complete Cap0 structure m7Gppp5´N to mRNA (Figure 1b). This product can be applied to capping reaction of RNA generated by the T7 RNA Polymerase (GMP-E121, Novoprotein). The capping reaction is completed within an hour and the efficiency is close to 100% with correct direction. Vaccinia Capping Enzyme is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification. Figure 1. The structure and mechanism of vaccinia virus capping. a. Co-crystal structure of vaccinia virus capping enzyme and GTP (red), SAH (rose red) (PDB 4CKB). The enzyme consists of two subunits, D1 and D12, and has the functions of RNA triphosphatase (blue), guanosine transferase (orange) and guanine methyltransferase activities (beige). This figure is quoted from Ramanathan, A., Robb, GB, & Chan, SH (2016). mRNA capping: biological functions and applications. Nucleic Acids Res 44 (16), 7511–7526. b. The cap structure of mRNA is composed of a 7-Methylguanosine consists of a 5’-5’ triphosphate bridge connected to the 5’ nucleoside of the mRNA chain. The Cap-0 structure is formed by the sequential reaction of three enzymes between adjacent RNA strands. Further formation of cap-1 structure requires the participation of 2-O methyltransferase. This modification can reduce the cellular innate immune response caused by RNA in vivo. This figure is quoted from Decroly, E., Ferron, F., Lescar, J. et al. (2012). Conventional and unconventional mechanisms for capping viral mRNA. Nat Rev Microbiol 10, 51–65.