BisulFlash DNA Modification Kit

A complete set of optimized buffers and reagents to perform DNA modification using an expedited DNA bisulfite conversion technology developed by Epigentek, geared specifically for methylation-specific PCR (MSP) as well as post-bisulfite based NGS applications. Through a proprietary composition which allows DNA denaturation and bisulfite conversion to be processed at the same time, the complete procedure is reduced to only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil. Perfecting Bisulfite Conversion Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step -- but with the BisulFlash method, DNA denaturation status is concurrently sustained throughout the entire DNA bisulfite conversion process. This breakthrough approach enables the DNA conversion process to be significantly faster with higher conversion efficiency and accuracy. We continue to innovate with the development of the new BisulFlash kit by identifying four critical components of bisulfite conversion: • Speed: Reduce the entire procedure to as short as 30 minutes without any reagent setup time. • Efficiency: Completely convert unmethylated cytosine into uracil -- modified DNA > 99.9%. • DNA Protection: Protect against DNA degradation of which more than 90% of DNA loss can be prevented, allowing for greater recovery. • Sensitivity: Start with the lowest amount of input DNA for modification -- only 0.2 ng or just 50 cells.