pMEV2HA-AKT1-AA

pMEV2HA-AKT1-AA is a high-quality, expression-ready plasmid encoding a dominant-negative mutant of human AKT1, where key phosphorylation sites Thr308 and Ser473 are substituted with alanine (T308A/S473A). This dual mutation renders the AKT1 protein inactive, preventing activation by upstream kinases, making it a powerful tool for studying AKT1 signaling pathways, apoptosis regulation, and growth factor response. Expressed under the pMEV2 promoter with an N-terminal HA tag for easy detection, this clone is ideal for transfection into mammalian cells to suppress endogenous AKT1 activity and analyze phosphorylation-dependent cellular processes. - 20 ug