ELISA SFN anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:14-3-3 proteins regµLate many cellµLar processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regµLating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), throµgh recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimµLate the Akt/mTOR signaling pathway by upregµLating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regµLates the tumor suppressor p53 and increases the rate of p53-regµLated inhibition of G2/M cell cycle progression. MµLtiple isoforms of 14-3-3 s exist due to alternative splicing events. Immunogen:14-3-3 protein sigma Synonyms:14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS Observed MW:31 kDa Uniprot ID:P31947 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IHC, IF Recommended dilution:WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100 Gene ID:2810 Research Area:Neuroscience, Signal Transduction