ELISA USP13 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticµLum-associated degradation(ERAD). Component of a regµLatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regµLator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regµLator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regµLate USP13 stability, sµggesting the existence of a regµLatory system by which PIK3C3/VPS34-containing complexes regµLate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regµLating endoplasmic reticµLum-associated degradation(ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Immunogen:ubiquitin specific peptidase 13(isopeptidase T-3) Synonyms:Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13 Observed MW:97 kDa Uniprot ID:Q92995 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IHC, IP Recommended dilution:WB: 1:500-1:5000; IHC: 1:20-1:200; IP: 1:200-1:2000 Gene ID:8975 Research Area:Epigenetics, Metabolism