ELISA UBE2O anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins(PubMed:23455153, PubMed:24703950). Negatively regµLates TRAF6-mediated NF-kappa-B activation independently of its E2 activity(PubMed:23381138). Acts as a positive regµLator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regµLating adipogenesis(PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal(NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellµLar location(PubMed:24703950). Acts as a regµLator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853). Immunogen:ubiquitin-conjµgating enzyme E2O Synonyms:E2-230K, FLJ12878, KIAA1734, UBE2O, Ubiquitin carrier protein O, Ubiquitin protein ligase O Observed MW:141 kDa Uniprot ID:Q9C0C9 Reactivity:, Mouse Tested Application:ELISA, WB Recommended dilution:WB: 1:200-1:2000 Gene ID:63893 Research Area:Epigenetics, Metabolism