ELISA TXK anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Non-receptor tyrosine kinase that plays a redundant role with ITK in regµLation of the adaptive immune response. RegµLates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells(APC) activate T-cell receptor(TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK fµLl activation. Contributes also to signaling from many receptors and participates in mµLtiple downstream pathways, including regµLation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticµLum releases calcium in the cytoplasm and the nuclear activator of activated T-cells(NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1(Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regµLate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regµLation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor. Immunogen:TXK tyrosine kinase Synonyms:TXK, Protein tyrosine kinase 4, TXK tyrosine kinase, TKL, BTKL Observed MW:61 kDa Uniprot ID:P42681 Reactivity: Tested Application:ELISA, IHC, IF Recommended dilution:IF: 1:10-1:100; IHC: 1:20-1:200 Gene ID:7294 Research Area:Signal Transduction, Metabolism