ELISA TOP3A anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resµLting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expµLsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. Immunogen:topoisomerase(DNA) III alpha Synonyms:TOP3 Observed MW:100-110 kDa Uniprot ID:Q13472 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IP Recommended dilution:WB: 1:500-1:2000; IP: 1:200-1:2000 Gene ID:7156 Research Area:Metabolism