ELISA SCAP anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Escort protein required for cholesterol as well as lipid homeostasis. RegµLates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regµLation of LDLR, INSIG1 and the mevalonate pathway(By similarity).SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770). Immunogen:SREBF chaperone Synonyms:KIAA0199 Observed MW:140 kDa,96 kDa Uniprot ID:Q12770 Reactivity:, Mouse Tested Application:ELISA, WB, IHC, IF Recommended dilution:WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 Gene ID:22937 Research Area:Metabolism