ELISA TransDetect® Cell Counting (CCK)

Product Details TransDetect® Cell Counting Kit (CCK) is designed for cell proliferation assays as well as cytotoxicity assays by utilizing a water-soluble tetrazolium salt. The salt can be reduced to an orange water-soluble formazan by mitochondrial dehydrogenase in the presence of an electron coupling reagent 1-Methoxy PMS. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. Faster cell proliferation, lower cytotoxicity, and more cell number produce deeper color. The depth of the dye is directly proportional to the number of living cells. The toxicity of CCK solution is so low that the same cells can be used for other assays after the CCK assay is completed. Compared with MTT, XTT, MST and WST-1, this method provides higher sensitivity and broader linear range. It is suitable for drµg screening, cell proliferation test, cytotoxicity assay and drµg sensitivity test. Storage at 2-8℃ in dark for one year or at -20℃ in dark for two years. Shipping Dry ice (-70℃) Citations Li P, Du J, Goodier JL, et al. Aicardi-Goutières syndrome protein TREX1 suppresses L1 and maintains genome integrity throµgh exonuclease-independent ORF1p depletion.Nucleic Acids Res. 2017,45(8):4619-4631. Gu J , Wang D , Zhang J , et al. GFRα2 prompts cell growth and chemoresistance throµgh down-regµLating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer[J]. Cancer Letters, 2016, 380(2):434-441. Li J , Zhuang C , Liu Y , et al. Synthetic tetracycline-controllable shRNA targeting long non-coding RNA HOXD-AS1 inhibits the progression of bladder cancer[J]. Journal of Experimental & Clinical Cancer Research, 2016, 35(1):99. Zhigang Zhu,a Jianliang Zhou, Jingli Ding, et al. A novel approach to prepare a tissue engineering decellµLarized valve scaffold with poly(ethylene glycol)–poly(ε-caprolactone)[J]. RSC Adv. 2016, 6(17):14427-14438. Min Q H , Wang X Z , Zhang J , et al. Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drµg-resistant trait by delivering miR-365[J]. Experimental Cell Research, 2017, 362(2):386-393.