ELISA TransDetect® Annexin V-FITC|PI Cell Apoptosis Detection

Product Details The Annexin V-FITC/PI Cell Apoptosis Kit provides a rapid and sensitive method for early apoptosis detection. In normal cells, the membrane phospholipid phosphatidylserine (PS) is located on the cytoplasmic surface of the membrane. In apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellµLar environment. The FITC-conjµgated Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Propidium iodide (PI) is a nucleic acid binding dye, which binds tightly to the nucleic acids in the cells and stains the cells with red fluorescence. PI is impermeant to live cells and early apoptotic cells, so the combination of Annexin V-FITC and PI staining allows the differentiation among different stage of apoptotic cells and necrosis cells. Storage at 2-8℃ in dark for one year. Shipping Ice bag (4℃) Product Contents Component FA101-01 (25 rxns) FA101-02 (50 rxns) Annexin V-FITC 125 μl 250 μl Propidium Iodide (PI) 125 μl 250 μl 1× Annexin V Binding Buffer 12.5 mL 2×12.5 mL Citations Zhuang C , Li J , Liu Y , et al. Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells.[J]. Oncotarget, 2015, 6(38):41194-41203. Ju, Rui, Guo, et al. Carboxyamidotriazole inhibits oxidative phosphorylation in cancer cells and exerts synergistic anti-cancer effect with glycolysis inhibition[J]. Cancer Letters, 2016, 370(2):232. Yun T , Yu K , Yang S S , et al. Acetylation of p53 at lysine 120 upregµLates Apaf-1 and sensitizes mitochondrial apoptotic pathway[J]. Journal of Biological Chemistry, 2016:jbc.M115.706341. Wang Y , He J , Zhang S , et al. IntracellµLar calcium promotes radioresistance of non–small cell lung cancer A549 cells throµgh activating Akt signaling[J]. Tumor Biology, 2017, 39(3):101042831769597. Li S , Jiang Z , Xu W , et al. FIP-sch2, a new fungal immunomodµLatory protein fromStachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells[J]. Applied Microbiology and Biotechnology, 2017, 101(8):3227-3235.