ELISA Di-Methyl-Histone H4 (Arg3)|H4R3me2 anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4 GeneID:121504,554313,8294,8359,8360,8361,8362,8363,8364,8365,8366,8367,8368,8370 Uniprot:P62805 Specificity:Di-Methyl-Histone H4 (Arg3)/H4R3me2 Antibody detects endogenous levels of Di-Methyl-Histone H4 only when methylated at Arg3. Immunogen:A synthetic methylated peptide derived from Di-Methyl-Histone H4 around the methylation site of Arg3. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction: Application:WB,IF/ICC,IP,ChIP,ELISA(peptide) Dilution:WB 1:500-1:2000, IF/ICC 1:50-1:200, IP 1:50-1:100, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2838911 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:DF6954-BP Des# Blocking Peptide:Di-Methyl-Histone H4 (Arg3)/H4R3me2 Blocking peptide Blocking Peptide Price:$549 CAD