ELISA HiPure SF Plant DNA

20% OFF list price as part of our FALL Sale! On now until October 18th, 2024! Introduction This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction or alcohol precipitation and involves minimal handling. Details Specifications Features Specifications Main Functions Isolation total DNA from100 mg simple plant Applications PCR, SSR, AFLP, RAPD and southern blot, etc. Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifµgation or vacuum) Sample type Economic plant samples Sample amount Fresh / frozen plant samples: ≤100m gDried plant / seed samples: ≤20mg Elution volume ≥30μl Time per run 30-50 minutes Liquid carrying volume per column 800μl Binding yield of column 100μg Principle Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifµged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water. Advantages Safe - require no phenol or chloroform extraction Fast - several samples can be extracted in 30 minutes by silica technology High purity - high quality DNA, completely remove inhibitors High yield - silica technology can achieve the highest yield Kit Contents Contents D316402 D316403 Purification Times 50 Preps 250 Preps RNase A 10 mg 50 mg Protease Dissolve Buffer 1.8 mL 5 mL Buffer SPL 30 mL 150 mL Buffer PS 10 mL 50 mL Buffer GW1 22 mL 110 mL Buffer GW2* 12 mL 2 x 50 mL Buffer AE 15 mL 60 mL HiPure gDNA Mini Columns 50 2 x 125 2 mL Collection Tubes 50 2 x 125 Storage and Stability RNase A shoµLd be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.