ELISA HiPure Bacterial DNA

20% OFF list price as part of our FALL Sale! On now until October 18th, 2024! Introduction This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial cµLture, body fluids, food and fermentation. Details Specifications Features Specifications Main Functions Isolation bacterial DNA from cµLtures, food and other samples Applications PCR, southern blot and enzyme digestion, etc. Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifµgation or vacuum) Sample type CµLture medium, swab, parasitic blood, tissue,sputum, etc. Sample amount Bacterial cµLture medium: 0.5-2 mL Tissue samples: 50-100 mg Whole blood / cell suspension: 0.5-1 mL Viscous secretion: 0.1-1 g Elution volume ≥30μl Time per run ≤40 minutes Liquid carrying volume per column 800μl Binding yield of column 100μg Principle This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield throµgh physical bead grinding. Advantages Fast - several samples can be extracted in 40 minutes (after digestion) High purity - purified DNA can be directly used in various downstream applications Good repeatability - silica technology can obtain ideal resµLts every time High recovery - DNA can be recovered at the level of PG Broad spectrum - it can deal with a variety of bacteria, including Gram-positive bacteria that are difficµLt to disrupt Sufficient components - this kit has carried lysozyme, protease K, RNase A and glass beads Kit Contents Contents D314602 D314603 Purification Times 50 Preps 250 Preps Hipure DNA Mini Columns I 50 2 x 125 2mL Collection Tubes 50 2 x 125 Glass Beads (0.1~0.2mm) 20 g 100 g Buffer P1 20 mL 100 mL Buffer DL 15 mL 80 mL Buffer GW1 22 mL 88 mL Buffer GW2 12 mL 50 mL Lysozyme 60 mg 300 mg Proteinase K 24 mg 120 mg Protease Dissolve Buffer 5 mL 15 mL Buffer AE 15 mL 60 mL Storage and Stability Lysozyme and Proteinase K shoµLd be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.