ELISA Phospho-YAP1 (S127) anti-

Quantity :50µL Clone Number:3F3 Aliases:Transcriptional coactivator YAP1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1, YAP65 Product Type:Recombinant Antibody Immunogen Species: UniProt ID:P46937 Immunogen:A syntheQuantityd peptide derived from Phospho-YAP1 (S127) Raised in: Reactivity: Tested Applications:ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200 Background:Transcriptional regµLator which can act both as a coactivator and a corepressor and is the critical downstream regµLatory target in the Hippo signaling pathway that plays a pivotal role in organ Quantity control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regµLatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regµLatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regµLating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regµLate cellµLar genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimµLate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750). Clonality:Monoclonal Isotype:Rabbit IgG Purification Method:Affinity-chromatography Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:YAP1 Research Areas:Signal Transduction