ELISA SIAH1 anti-

Quantity :50µL Clone Number: Aliases:E3 ubiquitin-protein ligase SIAH1 antibody; FLJ08065 antibody; hSIAH1 antibody; HUMSIAH antibody; Seven in absentia homolog 1 (Drosophila) antibody; Seven in absentia homolog 1 antibody; Siah 1 antibody; Siah 1a antibody; Siah E3 ubiquitin protein ligase 1 antibody; Siah-1 antibody; Siah-1a antibody; Siah1 antibody; SIAH1_ antibody; SIAH1A antibody; Ubiquitin ligase SIAH1 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q8IUQ4 Immunogen:Recombinant E3 ubiquitin-protein ligase SIAH1 protein (1-313AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200 Background:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjµgating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either throµgh direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regµLation (ELL2, MYB, POU2AF1, PmL and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecµLes (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubµLe motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 throµgh proteasomal degradation. It is thereby involved in many cellµLar processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regµLation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimµLation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:SIAH1 Research Areas:Neuroscience; Cell biology