ELISA TRIP12 anti-

Quantity :50µL Clone Number: Aliases:TRIP12 antibody; KIAA0045 antibody; µLF antibody; E3 ubiquitin-protein ligase TRIP12 antibody; EC 2.3.2.26 antibody; E3 ubiquitin-protein ligase for Arf antibody; µLF antibody; HECT-type E3 ubiquitin transferase TRIP12 antibody; Thyroid receptor-interacting protein 12 antibody; TR-interacting protein 12 antibody; TRIP-12 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q14669 Immunogen:Recombinant E3 ubiquitin-protein ligase TRIP12 protein (1-240AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200 Background:E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regµLation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regµLated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regµLator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumµLation of \\\'Lys-63\\\'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:TRIP12 Research Areas:Epigenetics and Nuclear Signaling