ELISA GP anti-

Quantity :50µg Clone Number: Aliases:GP antibody; Envelope glycoprotein antibody; GP1,2 antibody; GP) [Cleaved into: GP1; GP2; GP2-delta] antibody Product Type:Polyclonal Antibody Immunogen Species:Zaire ebolavirus (strain Eckron-76) (ZEBOV) (Zaire Ebola virus) UniProt ID:P87671 Immunogen:Recombinant Zaire ebolavirus Envelope glycoprotein (502-637AA) Raised in:Rabbit Reactivity:Zaire ebolavirus Tested Applications:ELISA Background:GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection). Binding to the macrophage specific lectin CLEC10A also seems to enhance virus infectivity. Interaction with FOLR1/folate receptor alpha may be a cofactor for virus entry in some cell types, althoµgh resµLts are contradictory. Members of the Tyro3 receptor tyrosine kinase family also seem to be cell entry factors in filovirus infection. Once attached, the virions are internalized throµgh clathrin-dependent endocytosis and/or macropinocytosis. After internalization of the virus into the endosomes of the host cell, proteolysis of GP1 by two cysteine proteases, CTSB/cathepsin B and CTSL/cathepsin L presumably induces a conformational change of GP2, unmasking its fusion peptide and initiating membranes fusion. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:GP Research Areas:Others