ELISA Phospho-MDM2 (Ser166) anti-

Quantity :100µL Clone Number: Aliases:ACTFS antibody; Double minute 2 protein antibody; E3 ubiquitin-protein ligase Mdm2 antibody; Hdm 2 antibody; Hdm2 antibody; HDMX antibody; MDM 2 antibody; MDM2 antibody; MDM2 oncogene E3 ubiquitin protein ligase antibody; Mdm2 p53 E3 ubiquitin protein ligase homolog antibody; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody; MDM2_ antibody; MDM2BP antibody; Mouse Double Minute 2 antibody; MTBP antibody; Murine Double Minute Chromosome 2 antibody; Oncoprotein Mdm2 antibody; p53 Binding Protein Mdm2 antibody; p53-binding protein Mdm2 antibody; Ubiquitin protein ligase E3 Mdm2 antibody; Ubiquitin protein ligase E3 Mdm2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q00987 Immunogen:Peptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from MDM2. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 Background:This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregµLatory negative feedback loop. Overexpression of this gene can resµLt in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis throµgh interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues Haupt, Y. et al. (1997) Nature 387, 296-299. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:MDM2 Research Areas:Epigenetics and Nuclear Signaling;Cancer;Cell biology