ELISA Phospho-CHEK1 (Ser286) anti-

Quantity :100µL Clone Number: Aliases:C85740 antibody; Cell cycle checkpoint kinase antibody; Checkpoint ; S. pombe; homolog of; 1 antibody; Checkpoint kinase 1 antibody; Checkpoint kinase 1 homolog (S. pombe) antibody; CHEK 1 antibody; Chek1 antibody; Chk 1 antibody; Chk1 antibody; CHK1 checkpoint homolog (S. pombe) antibody; CHK1_ antibody; EC 2.7.11.1 antibody; rad27 antibody; Serine/threonine protein kinase Chk1 antibody; Serine/threonine-protein kinase CHK1 antibody; STT3; subunit of the oligosaccharyltransferase complex; homolog A (S. cerevisiae) antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:O14757 Immunogen:Peptide sequence around phosphorylation site of serine 286 (S-E-S(p)-P-S) derived from Chk1. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB; WB:1:500-1:1000 Background: Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regµLate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate mµLtiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellµLar proliferation. Enomoto M, et al. (2009)J Biol Chem 284, 34223-3 Ikegami Y, et al. (2008)Biochem Biophys Res Commun 377, 1227-31 Shiromizu T, et al. (2006)Genes Cells 11, 477-85 Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:CHEK1 Research Areas:Epigenetics and Nuclear Signaling;Cancer