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ELISA UBB anti-

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Quantity :50µL Clone Number: Aliases:UBB antibody; UBB_ antibody; Ubiquitin antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P0CG47 Immunogen:Recombinant Polyubiquitin-B protein (103-228AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200 Background:Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjµgated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticµLum-associated degradation) and in cell-cycle regµLation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjµgated to Lys residues of target proteins, however, in rare cases, conjµgation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Antigen Affinity purified & Affinity purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:UBB Research Areas:Signal Transduction

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