ELISA TNF anti-

Quantity :100µL Clone Number: Aliases:APC1 antibody; APC1 protein antibody; Cachectin antibody; DIF antibody; Differentiation inducing factor antibody; Macrophage cytotoxic factor antibody; Tnf antibody; TNF superfamily member 2 antibody; TNF superfamily, member 2 antibody; TNF, macrophage derived antibody; TNF, monocyte derived antibody; TNF-a antibody; TNF-alpha antibody; TNFA antibody; TNFA_ antibody; TNFSF2 antibody; Tumor necrosis factor (TNF superfamily member 2) antibody; Tumor necrosis factor alpha antibody; Tumor necrosis factor antibody; Tumor necrosis factor ligand superfamily member 2 antibody; Tumor Necrosis Factor, Membrane Form antibody; Tumor necrosis factor, soluble form antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P01375 Immunogen:C term -peptide of SFPQ Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB, IHC; WB:1:1000-1:2000, IHC:1:50-1:100 Background:TNF-?, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-? is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-? are biologically active. TNF-? is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. CellµLar response to TNF-? is mediated throµgh interaction with receptors TNF-R1 and TNF-R2 and resµLts in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-?B) promotes the survival of cells, while TNF-?-mediated activation of caspase-8 leads to programmed cell death. TNF-? plays a key regµLatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tubercµLosis. The role of TNF-? in autoimmunity is underscored by blocking TNF-? action to treat rheumatoid arthritis and Crohn’s disease. Clonality:Polyclonal Isotype:IgG Purification Method:Affinity purification Conjµgate:Non-conjµgated Buffer:Store at -20°C or -80°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:TNF Research Areas:Cancer?CardiovascµLar;Immunology?Metabolism;Signal transduction