ELISA SUPT4H1 anti-

Quantity :50µL Clone Number: Aliases:DRB sensitivity-inducing factor 14 kDa subunit antibody; DRB sensitivity-inducing factor small subunit antibody; DSIF p14 antibody; DSIF small subunit antibody; hSPT4 antibody; SPT4 antibody; SPT4H antibody; SPT4H_ antibody; SUPT4H `1 antibody; SUPT4H antibody; Supt4h1 antibody; Transcription elongation factor spt4 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P63272 Immunogen:Recombinant Transcription elongation factor SPT4 protein (1-117AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200 Background:Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regµLates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regµLates mRNA capping by stimµLating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimµLates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3\\\' terminus and may allow repeated attempts at transcription throµgh natural pause sites. DSIF can also positively regµLate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:SUPT4H1 Research Areas:Epigenetics and Nuclear Signaling