ELISA NR3C1 anti-

Quantity :100µL Clone Number: Aliases:GCCR antibody; GCR antibody; GCR_ antibody; GCRST antibody; glucocorticoid nuclear receptor variant 1 antibody; Glucocorticoid receptor antibody; GR antibody; GRL antibody; Grl1 antibody; nr3c1 antibody; Nuclear receptor subfamily 3 group C member 1 antibody; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P04150 Immunogen:Recombinant protein of NR3C1 Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB, IHC; WB:1:500-1:2000, IHC:1:50-1:200 Background:Glucocorticoid hormones control cellµLar proliferation, inflammation, and metabolism throµgh their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors. GR is composed of several conserved structural elements, including a carboxy-terminal ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization signals, a central zinc-finger-containing DNA-binding domain, and an amino-terminal variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant popµLation of GR is localized to the cytoplasm in an inactive form via its association with regµLatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific DNA sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes. It was demonstrated that GR-mediated transcriptional activation is modµLated by phosphorylation. Althoµgh GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been sµggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor signaling, receptor stability, and receptor subcellµLar localization. Indeed Ser211 of GR is phosphorylated to a greater extent in the presence of hormone, and biochemical fractionation studies following hormone treatment indicate that Ser211-phosphorylated GR is found in the nucleus. Thus, Ser211 phosphorylation is a biomarker for activated GR in vivo. An added layer of complexity to GR signaling lies in the ability of mµLtiple isoforms to be generated throµgh both alternative splicing and the use of alternative translation intiation start sites, thus increasing the repertoire of functional signaling homo- and heterodimers. Clonality:Polyclonal Isotype:IgG Purification Method:Affinity purification Conjµgate:Non-conjµgated Buffer:Store at -20°C or -80°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:NR3C1 Research Areas:Epigenetics and Nuclear Signaling;Cancer;Signal transduction