ELISA LEP Antibody, HRP

Quantity :50µg Clone Number: Aliases:LEP antibody; OB antibody; OBS antibody; Leptin antibody; Obesity factor antibody Product Type:Polyclonal Antibody Immunogen Species:Bos taurus (Bovine) UniProt ID:P50595 Immunogen:Recombinant Bovine Leptin protein (22-167AA) Raised in:Rabbit Reactivity:Bovine Tested Applications:ELISA Background:Key player in the regµLation of energy balance and body weight control. Once released into the circµLation, has central and peripheral effects by binding LEPR, found in many tissues, which resµLts in the activation of several major signaling pathways (By similarity). In the hypothalamus, acts as an appetite-regµLating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regµLates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regµLates pancreatic beta-cell function and insµLin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modµLatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (By similarity). Acts as a growth factor on certain tissues, throµgh the activation of different signaling pathways increases expression of genes involved in cell cycle regµLation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity). May also play an apoptotic role via JAK2-STAT3 pathway and up-regµLation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascµLar endothelial cells and plays a role in matrix remodeling by regµLating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modµLates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, throµgh a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (By similarity). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimµLation, throµgh MTOR signaling pathway activation and BCL2 up-regµLation (By similarity). Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:HRP Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:LEP Research Areas:Neuroscience; Cancer; CardiovascµLar; Metabolism; Signal transduction; Stem cells