ELISA TNF Monoclonal anti-

Quantity :50µg Clone Number:2E1A5 Aliases:Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2 Product Type:Monoclonal Antibody Immunogen Species:Bovine UniProt ID:Q06599 Immunogen:Recombinant Bovine TNF alpha protein Raised in:Mouse Reactivity:Bovine Tested Applications:ELISA Background:Tumor necrosis factor alpha (TNF?,also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecµLe that plays a central role in inflammation, apoptosis, and immune system development. TNF? is produced by a wide variety of immune and epithelial cell types. Bovine TNF? consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellµLar domain (ECD). Within the ECD, bovine TNF? shares 64%-83% sequence identity with canine, cotton rat, equine, feline, , mouse, porcine, rat, and rhesus TNF?.The 26 kDa type 2 transmembrane protein is assembled intracellµLarly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimµLation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF? by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF?. TNF? trimers bind the ubiquitous TNF RI and the hematopoietic cell restricted TNF RII, both of which are also expressed as homotrimers. TNF? regµLates lymphoid tissue development throµgh control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascµLar endothelial cells and macrophages. TNF? is a key cytokine in several inflammatory disorders. It contributes to the development of type 2 diabetes throµgh its effects on insµLin resistance and fatty acid metabolism. Clonality:Monoclonal Isotype:IgG2b Purification Method:>95%,Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:TNF Research Areas:Others