ELISA Flag Tag Monoclonal anti-

Quantity :50µL Clone Number:4G5C8 Aliases:DDK tag, DYKDDDDK, Flag, Flag Tag Product Type:Tag/Control Antibodies Immunogen Species: UniProt ID: Immunogen:DYKDDDDK(Flag) synthetic peptide conjµgate to KLH Raised in:Mouse Reactivity: Tested Applications:ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl Background:Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with mµLtiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellµLar localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Clonality:Monoclonal Isotype:IgG2a Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:Flag Tag Research Areas: