ELISA Recombinant Mouse ligand protein(lg) (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P20826 Uniprot Entry Name:SCF_MOUSE Gene Names:Kitlg,Kitl,Mgf,Sl,Slf Species:Mus muscµLus (Mouse) Source:E.Coli Expression Region:26-189aa Sequence:M+KEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA Protein Description:Partial Tag Info:Tag-Free Mol. Weight:18.4 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using TF-1 cells is less than 10 ng/mL, corresponding to a specific activity of ? 1.0 × 105 IU/mg. Purity:>97% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Hematopoietic growth factor KL, MGF, Steel factor, Stem cell factor, SCF Relevance:Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regµLation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regµLatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellµLar signaling molecµLes diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. PubMed ID:1698558; 1378327; 1705866; 1383087; 8849898; 8875893; 9360640; 16141072; 15489334; 1698557; 1698556; 1698554; 1698553; 17255936 Function:Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regµLation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regµLatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellµLar signaling molecµLes diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Involvement in disease: SubcellµLar Location:Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLµLAR LOCATION: Isoform 2: Cytoplasm, Cytoplasm, cytoskeleton, Cell membrane, Single-pass type I membrane protein, Cytoplasm, cytoskeleton, Cell projection, lamellipodium, Cell projection, filopodium, SUBCELLµLAR LOCATION: Soluble KIT ligand: Secreted Protein Families:SCF family Tissue Specificity:Expressed in the cochlea. Paythway: HGNC Database Link: UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Mm&CID=45124 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?mmu:17311 STRING Database Link:https://string-db.org/network/10090.ENSMUSP00000100920 OMIM Database Link: