ELISA Trans5alpha Chemically Competent Cell
Supplied in 2 x 50μl reactions per vial; 100μl per vial.
20 reaction Quantity is delivered in 10 vials; 40 reaction Quantity is delivered in 20 vials.
Description
Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).
Genotype
F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk-, mk+) supE44λ- thi-1 gyrA96relA1 phoA
Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• Reduced recombination of cloned DNA.
• Blue/white selection.
Storage
Competent Cell at -70°C for six months; others at -20°C for one year
Notes
Higher efficiency transformation can be achieved by transforming cells immediately following thawing.
Avoid repeated thawing.
Gentle handling is required for the entire procedure.
References:
Literature Journal IF Author Date Link
Fungicides induced triazole-resistance in Aspergillus fumigatus associated with mutations of TR46/Y121F/T289A and its appearance in agricµLtural fields Journal of Hazardous Materials 6.065 Jingbei Ren,et al. 2017Mar The original link
Automatic Enumeration of Gold Nanomaterials at the Single-Particle Level Anal Chem 5.886 Xiao Xu, et al. 2015 Feb The original link
Utilization of biodiesel by-product as substrate for high-production of ??-farnesene via relatively balanced mevalonate pathway in Escherichia coli Bioresource Technology 5.65 Shengping You et al 2017 Jun The original link
The antitumor activity and preliminary modeling on the potential mechanism of action of peroxiredoxin-5 Oncotarget 5.1657 Juanjuan Liu,et al. 2017Mar The original link
Monitoring and evaluation of antibiotic resistance genes in four municipal wastewater treatment plants in Harbin, Northeast China Environ Pollut 5.0989 Wen Q, et al. 2016 Jan The original link
Internal Reference:
CD201-01
Website URL:
/shop/cd201-01-elisa-trans5alpha-chemically-competent-cell-178240
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