ELISA TransScript® II Green One-Step qRT-PCR SuperMix

20% OFF list price for the 400 rxn AND 1200 rxn variant as part of our FALL Sale! On now until October 18th, 2024! TransScript® II Green One-Step qRT-PCR SuperMix AQ311 is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly syntheQuantitys first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the syntheQuantityd cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube. Highlights • TransScript® II Green One-Step RT/RI Enzyme Mix is used to efficiently syntheQuantity first-strand cDNA from RNA and 2×PerfectStartTM Green One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination. • High sensitivity and high specificity to ensure accurate resµLts. Applications • High-copy and low-copy gene detection • Detection of RNA virus or trace amounts of RNA Storage at -20°C away from light for two years Shipping Dry ice (-70 ℃) Product Contents 100rxn x 20µL 400rxn x 20µL 1000rxn x 20µL Component AQ311-01 AQ311-02 AQ311-03 TransScript® II Green One-Step RT/ RI Enzyme Mix 40 μl 160 μl 400 μl PerfectStart® Green One-Step qPCR SuperMix (2×) 1 mL 4×1 mL 10×1 mL Passive Reference Dye (50×) 40 μl 160 μl 400 μl RNase-free Water 1 mL 4×1 mL 10×1 mL Citations Synaptotagmin-11 inhibits cytokine secretion and phagocytosis in microglia[J]. Glia, 2017, 65(10):1656-1667. A differential sequencing-based analysis of the C. elegans noncoding transcriptome[J]. RNA, 2012, 18(4):626-639. RegµLatory elements of Caenorhabditis elegans ribosomal protein genes[J]. BMC Genomics, 2012, 28(13):433.4.The loss of MiR-139-5p promotes colitis-associated tumorigenesis by mediating PI3K/AKT/Wnt signaling[J]. The International Journal of Biochemistry & Cell Biology, 2015, 69:153-161. Identification of Intermediate-Quantity Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans[J]. PLoS ONE, 2012, 7(11):e48066.