ELISA Acetyl-Histone H3 (Lys9|14|18|23|27) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:HIST2H3A; HIST2H3C; HIST2H3D,HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J,H3F3A; H3F3B GeneID:126961,333932,653604,8350,8351,8352,8353,8354,8355,8356,8357,8358,8968,3020,3021 Uniprot:P68431/Q71DI3/P84243 Specificity:Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody detects endogenous levels of Acetyl-Histone H3 only when acetylated at Lys9/14/18/23/27. Immunogen:A syntheQuantityd peptide derived from Histone H3 around the acetylation site of Lys9/14/18/23/27. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction:Bovine Application:WB,IHC,IF/ICC,ELISA(peptide) Dilution:WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2844627 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF4365-BP Des# Blocking Peptide:Acetyl-Histone H3 (Lys9/14/18/23/27) Blocking peptide Blocking Peptide Price:$549 CAD