ELISA Fish Na+|K+-ATPase (Na|K ATPase)

Reactivity:Fish UniProt:N/A Abbreviation:Na/K ATPa Alternative Names:N/A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.1% Inter-AssayCV:?8.3% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate Na/K ATPa in samples. An antibody specific for Na/K ATPa has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNa/K ATPa present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for Na/K ATPa is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Na/K ATPa bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Sodium/potassium-transporting ATPase subunit beta-3 belongs to the family of Na /K and H /K ATPases beta chain proteins, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregµLation, for sodium-coupled transport of a variety of organic and inorganic molecµLes, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regµLates, throµgh assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).