ELISA Dual specificity mitogen-activated protein kinase kinase 5 (MAP2K5)

Reactivity: (Homo sapiens) UniProt:Q13163 Abbreviation:MAP2K5 Alternative Names:HsT17454; MAPKK5; MEK5; PRKMK5; MAP kinase kinase MEK5b|MAPK/ERK kinase 5|dual specificity mitogen-activated protein kinase kinase 5 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.5% Inter-AssayCV:?8.7% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MAP2K5 in samples. An antibody specific for MAP2K5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP2K5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MAP2K5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP2K5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dual specificity mitogen-activated protein kinase kinase 5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5.This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimµLated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.Expressed in many adµLt tissues. Abundant in heart and skeletal muscle. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).