ELISA Potassium intermediate|small conductance calcium-activated channel, subfamily N, member 2 (KCNN2)

Reactivity: (Homo sapiens) UniProt:Q9H2S1 Abbreviation:KCNN2 Alternative Names:KCa2.2; SK2; SKCA2; hSK2; apamin-sensitive small-conductance Ca2+-activated potassium channel|small conductance calcium-activated potassium channel protein 2 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?4.2% Inter-AssayCV:?7.8% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KCNN2 in samples. An antibody specific for KCNN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KCNN2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNN2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The KCa2.2 protein is activated before membrane hyperpolarization and is thoµght to regµLate neuronal excitability by contributing to the slow component of synaptic AHP. KCa2.2 is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodµLin-binding subunits. KCNN2 is a member of the calcium-activated potassium channel family. Two transcript variants encoding different isoforms have been found for the KCNN2 gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).