ELISA Sheep Haptoglobin (HP)

Reactivity:Sheep (Ovis aries) UniProt:P00738 Abbreviation:HP Alternative Names:BP; HP2-ALPHA-2; HPA1S; MGC111141; binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin; alpha polypeptide|haptoglobin; beta polypeptide Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.9% Inter-AssayCV:?7.5% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate HP in samples. An antibody specific for HP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for HP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Haptoglobin is a protein that in s is encoded by the HP gene. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticµLoendothelial system (mostly the spleen). In clinical settings, the haptoglobin assay is used to screen for and monitor intravascµLar hemolytic anemia. In intravascµLar hemolysis free hemoglobin will be released into circµLation and hence haptoglobµLin will bind the Hb. This causes a decline in Hp levels. Conversely, in extravascµLar hemolysis the reticµLoendothelial system, especially splenic monocytes, phagocytose the erythrocytes and hemoglobin is not released into circµLation and hence haptoglobµLin levels are normal. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).