ELISA Sheep Interleukin 10 (IL-10)

Reactivity:Sheep (Ovis aries) UniProt:Q29408 Abbreviation:IL10 Alternative Names:CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.1% Inter-AssayCV:?7.8% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate IL10 in samples. An antibody specific for IL10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for IL10 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Interleukin-10 is a small protein known as a cytokine that functions as an important regµLator of the immune system. Althoµgh IL-10 is known to have many different roles in the immune system, its two major activities include inhibition of cytokine production by macrophages and inhibition of the accessory functions of macrophages during T cell activation. The effects of these actions cause IL-10 to play mainly an anti-inflammatory role in the immune system. IL-10 was originally known as the cytokine synthesis inhibiting factor (CSIF), and the discovery of this protein was based on its biological activity. IL-10 is mainly produced by the Th2 subset of CD4+ helper cells. However, it is also produced by some activated B cells, some Th1 cells , activated macrophages, and some nonhematopoietic sources Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).