ELISA Sheep Interleukin 13 (IL13)

Reactivity:Sheep (Ovis aries) UniProt:N/A Abbreviation:IL13 Alternative Names:ALRH; BHR1; IL-13; MGC116786; MGC116788; MGC116789; P600 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.9% Inter-AssayCV:?10.1% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate IL13 in samples. An antibody specific for IL13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for IL13 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL13 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Interleukin 13 (IL-13) is produced primarily by activated T lymphocytes. It shows approximately 30% amino acid (aa) sequence identity with IL-4 and shares many biological properties with IL-4. IL-13 has mµLtiple effects on the differentiation and functions of monocytes/macrophages. It can suppress the cytotoxic functions of monocytes/macrophages and the production of proinflammatory cytokines by monocytes/macrophages. The biological effects of IL-13 are mediated by specific high-affinity cell surface receptor complexes. The functional IL-13 receptor complex has been shown to consist of the low-affinity IL-13 receptor ? chain and the IL-4 receptor ? subunit (IL-4 R?). This IL-13 receptor complex may also serve as an alternate high-affinity IL-4 receptor complex in IL-4 responsive cells that lack the ? chain. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).