ELISA Rat Myelin protein zero IgG anti-(MPZ-Ab-IgG)

Reactivity:Rat (Rattus norvegicus) UniProt:N/A Abbreviation:MPZ-Ab-IgG Alternative Names:N/A Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.122 ng/mL Intra-AssayCV:?5.5% Inter-AssayCV:?9.9% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Qualitative Test principle:This assay employs a two-site sandwich ELISA to quantitate MPZ-Ab-IgG in samples. An antibody specific for MPZ-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPZ-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MPZ-Ab-IgG is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPZ-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Myelin protein-zero is the major structural protein of peripheral myelin.Hayasaka et al. (1993) stated that the MPZ gene contains 6 exons.You et al. (1991) found that the Mpz gene in the mouse, like that in the rat, contains 6 exons that span about 7 kb of genomic DNA.Myelin protein-zero is the major structural protein of peripheral myelin, accounting for more than 50% of the protein present in the sheath of peripheral nerves. Expression of the MPZ gene is restricted to Schwann cells; MPZ is not found in the CNS. An integral membrane glycoprotein of 28 kD, MPZ is thoµght to link adjacent lamellae and thereby stabilize the myelin assembly. The other 3 major components of myelin are myelin basic protein (MBP), myelin proteolipid protein (PLP1), and myelin-associated glycoprotein (MAG). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).